Lab worksheet
Lab 14 – How big are the objects when viewed on the scope

Submit to Lab 14 dropbox as Lab 14-week10_LastName_FirstInital

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Title:

Purpose:

Methods:

When looking down the microscope through the ocular lens you see the ruler at 40X magnification

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Count the number of mm spaces you can see and put that number here ____________

That number is the FOV measurement at 40X magnification

You will now use the formula listed below in results to calculate the FOV for 100X, 400X and 1000X total magnifications.

Results:

FOV calculations

FOV in mm

FOV in mm

4X -measured (M1)

D1 =

100X -calculated*(M2)

D2 =

400X -calculated*(M3)

D3 =

1000X – calculated (M4)

D4 =

To get 100X FOV use the formula M1*D1=M2*D2

Solve for D2

To get 400X FOV use the formula M1*D1=M3*D3

Solve for D3

To get 1000X FOV use the formula M1*D1=M4*D4

Solve for D4

Show your math

Conclusion:

What happens to the FOV as total magnification increases?

The prokaryotic organism Staphylococcus aureus measures 1 mm in diameter how many bacteria lined up across the diameter of the 1000X total magnification would fit?

The eukaryotic organism Paramecium measures 1 mm in length how many organisms lined up across the diameter of the 1000X total magnification would fit?

Hint think about size of object.